Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) ( ab150077 ). The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. A synthetic phosphopeptide derived from human Paxillin around the phosphorylation site of tyrosine 118 (H-V-Y P -S-F). Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). We use cookies to make our site as useful as possible. Visit protocols and troubleshooting or check them out using the Abcam app for iPhone. pH: 7.40 Preservative: 0.02% Sodium azide Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS Without Mg2+ and Ca2+. ab75740 at 1/50 dilution staining Paxillin in human breast carcinoma by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising phosphopeptide. Clinical success doesn't happen in isolation. Get access to the best antibodies, discovery platforms, and know-how to advance your diagnostic and therapeutic programs. Lane 1: HeLa cell extract, untreated Lane 2: HeLa cell extract treated with EGF. For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome. If incorrect, please enter your location into the box below, to view site information related to your location. Get resources and offers direct to your inbox. Your browser does not have JavaScript enabled and some parts of this website will not work without it. ab75740 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.. 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